Direct limits of infinite-dimensional Lie groups compared to direct limits in related categories

Let G be a Lie group which is the union of an ascending sequence of Lie groups G_n (all of which may be infinite-dimensional). We study the question when G is the direct limit of the G_n's in the category of Lie groups, topological groups, smooth manifolds, resp., topological spaces. Full answers are obtained for G the group Diff_c(M) of compactly supported smooth diffeomorphisms of a sigma-compact smooth manifold M, and for test function groups C^infty_c(M,H) of compactly supported smooth maps with values in a finite-dimensional Lie group H. We also discuss the cases where G is a direct limit of unit groups of Banach algebras, a Lie group of germs of Lie group-valued analytic maps, or a weak direct product of Lie groups.Comment: extended preprint version, 66 page

Perceived consequences of female labor-force participation: a multilevel latent-class analysis across 22 countries (Consecuencias percibidas de la participación femenina en el mercado de trabajo: un análisis multinivel de clases latentes en 22 países)

This paper investigates whether there are different patterns of traditionality in different countries with regard to a perceived negative impact of labor-force participation of mothers on their children and family life. For this purpose, individual-level traditionality subgroups and segments of countries with different traditionality patterns of their nationals were identified simultaneously by means of multilevel latent-class (ML-LC) analysis of the answers to three items of the Changing Family and Gender Roles module of the International Social Survey Program (ISSP). This module was fielded in 22 countries in the years 1994 and 2002. Six individual-level subgroups and five country segments can be discerned. The structure of individual-level subgroups is almost identical in both years. Four individual-level subgroups differ only quantitatively in their level of traditionality. Two further subgroups are characterized by a unique tendency to defend working mothers against criticism. From 1994 to 2002 the sizes of traditional subgroups decrease, and there is also some change in the composition of country segments. This paper investigates whether there are different patterns of traditionality in different countries with regard to a perceived negative impact of labor-force participation of mothers on their children and family life. For this purpose, individual-level traditionality subgroups and segments of countries with different traditionality patterns of their nationals were identified simultaneously by means of multilevel latent-class (ML-LC) analysis of the answers to three items of the Changing Family and Gender Roles module of the International Social Survey Program (ISSP). This module was fielded in 22 countries in the years 1994 and 2002. Six individual-level subgroups and five country segments can be discerned. The structure of individual-level subgroups is almost identical in both years. Four individual-level subgroups differ only quantitatively in their level of traditionality. Two further subgroups are characterized by a unique tendency to defend working mothers against criticism. From 1994 to 2002 the sizes of traditional subgroups decrease, and there is also some change in the composition of country segments.Este trabajo analiza si existen diferentes patrones de tradicionalismo en los diferentes países sobre la percepción de un impacto negativo de la participación laboral de las madres en sus hijos y en su vida familiar. Para ello se identificaron, de forma simultánea, subgrupos de tradicionalismo a nivel individual y segmentos de países con diferentes patrones de tradicionalismo de sus ciudadanos, a través de un análisis multinivel de clases latentes (ML-LC) de las respuestas a tres preguntas del módulo Familia y Roles de Género del International Social Survey Program (ISSP), que se aplicó en 22 países en los años 1994 y 2002. Se identificaron seis subgrupos de nivel individual y cinco segmentos de países. La estructura de los subgrupos a nivel individual es casi idéntica en ambos años. Cuatro subgrupos a nivel individual difieren solamente de manera cuantitativa en su nivel de tradicionalismo. Dos subgrupos más se caracterizan por una tendencia a defender de la crítica a las madres que trabajan. De 1994 a 2002 el tamaño de los subgrupos tradicionales disminuye, y también se ha producido algún cambio en la composición de los segmentos de países

Go with the Flow - Design of Cloud Logistics Service Blueprints

By adopting principles of cloud computing to the \ logistics domain the paradigm of Cloud Logistics is derived. It \ appears to be a promising paradigm in order to evolve logistics \ into being more flexible and collaborative. Yet, appropriate \ concepts that enable the cloud logistics paradigm are missing. \ In the paper, existing body of literature is reviewed and a \ definition and a framework of cloud logistics is given. Further, \ service blueprinting is combined with domain engineering and \ general morphological analysis in order to create a suitable \ method for designing cloud oriented service blueprints. Those \ are focusing on domain-specific flows and transformations \ enabling cloud oriented business collaboration. The method \ is applied to the logistics domain and a cloud logistics service \ blueprint is designed. Finally, the concept is evaluated with \ real use cases from logistics service providers

The ancestor of the Paulinella chromatophore obtained a carboxysomal operon by horizontal gene transfer from a Nitrococcus-like γ-proteobacterium

<p>Abstract</p> <p>Background</p> <p><it>Paulinella chromatophora </it>is a freshwater filose amoeba with photosynthetic endosymbionts (chromatophores) of cyanobacterial origin that are closely related to free-living <it>Prochlorococcus </it>and <it>Synechococcus </it>species (PS-clade). Members of the PS-clade of cyanobacteria contain a proteobacterial form 1A RubisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) that was acquired by horizontal gene transfer (HGT) of a carboxysomal operon. In rDNA-phylogenies, the <it>Paulinella </it>chromatophore diverged basal to the PS-clade, raising the question whether the HGT occurred before or after the split of the chromatophore ancestor.</p> <p>Results</p> <p>Phylogenetic analyses of the almost complete rDNA operon with an improved taxon sampling containing most known cyanobacterial lineages recovered the <it>Paulinella </it>chromatophore as sister to the complete PS-clade. The sequence of the complete carboxysomal operon of <it>Paulinella </it>was determined. Analysis of RubisCO large subunit (<it>rbcL</it>) sequences revealed that <it>Paulinella </it>shares the proteobacterial form 1A RubisCO with the PS-clade. The γ-proteobacterium <it>Nitrococcus mobilis </it>was identified as sister of the <it>Paulinella </it>chromatophore and the PS-clade in the RubisCO phylogeny. Gene content and order in the carboxysomal operon correlates well with the RubisCO phylogeny demonstrating that the complete carboxysomal operon was acquired by the common ancestor of the <it>Paulinella </it>chromatophore and the PS-clade through HGT. The carboxysomal operon shows a significantly elevated AT content in <it>Paulinella</it>, which in the <it>rbcL </it>gene is confined to third codon positions. Combined phylogenies using <it>rbcL </it>and the rDNA-operon resulted in a nearly fully resolved tree of the PS-clade.</p> <p>Conclusion</p> <p>The HGT of the carboxysomal operon predated the divergence of the chromatophore ancestor from the PS-clade. Following HGT and divergence of the chromatophore ancestor, diversification of the PS-clade into at least three subclades occurred. The γ-proteobacterium <it>Nitrococcus mobilis </it>represents the closest known relative to the donor of the carboxysomal operon. The isolated position of the <it>Paulinella </it>chromatophore in molecular phylogenies as well as its elevated AT content suggests that the <it>Paulinella </it>chromatophore has already undergone typical steps in the reductive evolution of an endosymbiont.</p

EST analysis of the scaly green flagellate Mesostigma viride (Streptophyta): Implications for the evolution of green plants (Viridiplantae)

BACKGROUND: The Viridiplantae (land plants and green algae) consist of two monophyletic lineages, the Chlorophyta and the Streptophyta. The Streptophyta include all embryophytes and a small but diverse group of freshwater algae traditionally known as the Charophyceae (e.g. Charales, Coleochaete and the Zygnematales). The only flagellate currently included in the Streptophyta is Mesostigma viride Lauterborn. To gain insight into the genome evolution in streptophytes, we have sequenced 10,395 ESTs from Mesostigma representing 3,300 independent contigs and compared the ESTs of Mesostigma with available plant genomes (Arabidopsis, Oryza, Chlamydomonas), with ESTs from the bryophyte Physcomitrella, the genome of the rhodophyte Cyanidioschyzon, the ESTs from the rhodophyte Porphyra, and the genome of the diatom Thalassiosira. RESULTS: The number of expressed genes shared by Mesostigma with the embryophytes (90.3 % of the expressed genes showing similarity to known proteins) is higher than with Chlamydomonas (76.1 %). In general, cytosolic metabolic pathways, and proteins involved in vesicular transport, transcription, regulation, DNA-structure and replication, cell cycle control, and RNA-metabolism are more conserved between Mesostigma and the embryophytes than between Mesostigma and Chlamydomonas. However, plastidic and mitochondrial metabolic pathways, cytoskeletal proteins and proteins involved in protein folding are more conserved between Mesostigma and Chlamydomonas than between Mesostigma and the embryophytes. CONCLUSION: Our EST-analysis of Mesostigma supports the notion that this organism should be a suitable unicellular model for the last flagellate common ancestor of the streptophytes. Mesostigma shares more genes with the embryophytes than with the chlorophyte Chlamydomonas reinhardtii, although both organisms are flagellate unicells. Thus, it seems likely that several major physiological changes (e.g. in the regulation of photosynthesis and photorespiration) took place early during the evolution of streptophytes, i.e. before the transition to land

First Impressions are More Important than Early Intervention Qualifying Broken Windows Theory in the Lab

Broken Windows: the metaphor has changed New York and Los Angeles. Yet it is far from undisputed whether the broken windows policy was causal for reducing crime. In a series of lab experiments we put two components of the theory to the test. We show that first impressions and early punishment of antisocial behaviour are independently and jointly causal for cooperativeness. The effect of good first impressions and of early vigilance cannot be explained with, but adds to, participants’ initial level of benevolence. Mere impression management is not strong enough to maintain cooperation. Cooperation stabilizes if good first impressions are combined with some risk of sanctions. Yet if we control for first impressions, early vigilance only has a small effect. The effect vanishes over time.

JCoast – A biologist-centric software tool for data mining and comparison of prokaryotic (meta)genomes

Background Current sequencing technologies give access to sequence information for genomes and metagenomes at a tremendous speed. Subsequent data processing is mainly performed by automatic pipelines provided by the sequencing centers. Although, standardised workflows are desirable and useful in many respects, rational data mining, comparative genomics, and especially the interpretation of the sequence information in the biological context, demands for intuitive, flexible, and extendable solutions. Results The JCoast software tool was primarily designed to analyse and compare (meta)genome sequences of prokaryotes. Based on a pre-computed GenDB database project, JCoast offers a flexible graphical user interface (GUI), as well as an application programming interface (API) that facilitates back-end data access. JCoast offers individual, cross genome-, and metagenome analysis, and assists the biologist in exploration of large and complex datasets. Conclusion JCoast combines all functions required for the mining, annotation, and interpretation of (meta)genomic data. The lightweight software solution allows the user to easily take advantage of advanced back-end database structures by providing a programming and graphical user interface to answer biological questions. JCoast is available at the project homepage

Origin of land plants: Do conjugating green algae hold the key?

<p>Abstract</p> <p>Background</p> <p>The terrestrial habitat was colonized by the ancestors of modern land plants about 500 to 470 million years ago. Today it is widely accepted that land plants (embryophytes) evolved from streptophyte algae, also referred to as charophycean algae. The streptophyte algae are a paraphyletic group of green algae, ranging from unicellular flagellates to morphologically complex forms such as the stoneworts (Charales). For a better understanding of the evolution of land plants, it is of prime importance to identify the streptophyte algae that are the sister-group to the embryophytes. The Charales, the Coleochaetales or more recently the Zygnematales have been considered to be the sister group of the embryophytes However, despite many years of phylogenetic studies, this question has not been resolved and remains controversial.</p> <p>Results</p> <p>Here, we use a large data set of nuclear-encoded genes (129 proteins) from 40 green plant taxa (Viridiplantae) including 21 embryophytes and six streptophyte algae, representing all major streptophyte algal lineages, to investigate the phylogenetic relationships of streptophyte algae and embryophytes. Our phylogenetic analyses indicate that either the Zygnematales or a clade consisting of the Zygnematales and the Coleochaetales are the sister group to embryophytes.</p> <p>Conclusions</p> <p>Our analyses support the notion that the Charales are not the closest living relatives of embryophytes. Instead, the Zygnematales or a clade consisting of Zygnematales and Coleochaetales are most likely the sister group of embryophytes. Although this result is in agreement with a previously published phylogenetic study of chloroplast genomes, additional data are needed to confirm this conclusion. A Zygnematales/embryophyte sister group relationship has important implications for early land plant evolution. If substantiated, it should allow us to address important questions regarding the primary adaptations of viridiplants during the conquest of land. Clearly, the biology of the Zygnematales will receive renewed interest in the future.</p

Megx.net—database resources for marine ecological genomics

Marine microbial genomics and metagenomics is an emerging field in environmental research. Since the completion of the first marine bacterial genome in 2003, the number of fully sequenced marine bacteria has grown rapidly. Concurrently, marine metagenomics studies are performed on a regular basis, and the resulting number of sequences is growing exponentially. To address environmentally relevant questions like organismal adaptations to oceanic provinces and regional differences in the microbial cycling of nutrients, it is necessary to couple sequence data with geographical information and supplement them with contextual information like physical, chemical and biological data. Therefore, new specialized databases are needed to organize and standardize data storage as well as centralize data access and interpretation. We introduce Megx.net, a set of databases and tools that handle genomic and metagenomic sequences in their environmental contexts. Megx.net includes (i) a geographic information system to systematically store and analyse marine genomic and metagenomic data in conjunction with contextual information; (ii) an environmental genome browser with fast search functionalities; (iii) a database with precomputed analyses for selected complete genomes; and (iv) a database and tool to classify metagenomic fragments based on oligonucleotide signatures. These integrative databases and webserver will help researchers to generate a better understanding of the functioning of marine ecosystems. All resources are freely accessible at